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Set C Index Adapters Confer with Index two (i5) Orientation For more info regarding how to enter i5 bases around the sample sheet in forward or reverse complement orientation.
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NextSeq 1000/2000 and NovaSeq X/X Moreover i5 indexes will properly be output from the ahead orientation for afterwards downstream processing. Custom made kits also needs to have the i5 indexes entered in the forward orientation.
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When employing BaseSpace Sequence Hub PrepTab, i5 sequences should be entered into these program inside the ahead orientation in all situations and for all instrument styles.
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